Publications
2026
Byrnes, N. A.; Silvester, R.; Cross, G.; Weightman, A. J.; Jones, D. L.; Kasprzyk-Hordern, B. A national-scale assessment of hospital contributions to chemical loads in wastewater and the implications for wastewater-based epidemiology. Environ. Int. 2026, 215, 110476. DOI: 10.1016/j.envint.2026.110476 . 
Byrnes, N. A.; Beaney, J.; Ceolotto, N.; Elabbadi, D.; Elliss, H.; Faill, B.; Hawkins, E.; Ranathunge, B.; Kasprzyk-Hordern, B. One Health water-based epidemiology: The chemical perspective. Trends Analyt. Chem. 2026, 204, 119051. DOI: 10.1016/j.trac.2026.119051.
Elliss, H.; Hricova, K.; Griffiths, E.; Byrnes, N.; Faill, B.; Hawkins, E.; Proctor, K.; Robertson, M.; Bagnall, J.; Kasprzyk-Hordern, B. Understanding the role of deconjugation of phase-II metabolites in wastewater: implications to Wastewater-Based Epidemiology. Environ. Sci. Technol. 2026, 60 (7), 5789–5803. DOI: 10.1021/acs.est.5c17466.
2025
Silvester, R.; Perry, W. B.; Webster, G.; Rushton, L.; Baldwin, A.; Pass, D. A.; Byrnes, N. A.; Farkas, K.; Heginbothom, M.; Craine, N.; et al. Metagenomic profiling of hospital wastewater: A comprehensive national scale analysis of antimicrobial resistance genes and opportunistic pathogens. J. Infect. 2025, 90 (6), 106503. DOI: 10.1016/j.jinf.2025.106503.
Byrnes, N. A.; Silvester, R.; Cross, G.; Weightman, A. J.; Jones, D. L.; Kasprzyk-Hordern, B. Assessing the risk of antimicrobial resistance and potential environmental harm through national-scale surveillance of antimicrobials in hospital and community wastewater. Environ. Int. 2025, 202, 109606. DOI: 10.1016/j.envint.2025.109606.
2024
Marriott, P. J.; Wong, Y. F.; Amaral, M.; Wu, Y.; Byrnes, N. A. Shape-shifting molecule behaviour during comprehensive two-dimensional gas chromatography analysis—A review. J. Chromatogr. Open 2024, 5, 100125. DOI: 10.1016/j.jcoa.2024.100125.
2023
Byrnes, N. A.; Wu, Y.; Nolvachai, Y.; Marriott, P. J. Exploring thermal isomerisation in gas chromatography analyses using natural pyrethrins: Comparison of comprehensive two-dimensional and one-dimensional gas chromatography. J. Chromatogr. A 2023, 1708, 464369. DOI: 10.1016/j.chroma.2023.464369.
2025
Kasprzyk-Hordern, B.; Byrnes, N. A.; Ceolotto, N.; Elliss, H.; Faill, B.; Hawkins, E.; Ranathunge, B. Chapter 22 – The anthropic fingerprinting of chemicals in wastewater: Wastewater-based epidemiology. Sample Handling and Trace Analysis of Pollutants (Second Edition); Barceló, D., Pico, Y. Eds.; Elsevier Science, 2025; pp 691–718. DOI: 10.1016/B978-0-323-85601-0.00034-5. 
2024
Silvester, R.; Perry, W. B.; Webster, G.; Rushton, L.; Baldwin, A.; Pass, D. A.; Byrnes, N.; Kille, P.; Farkas, K.; Kasprzyk-Hordern, B.; et al. A peek into hospital poo: Tracking pathogens and antimicrobial resistance. J. Glob. Antimicrob. Resist. 2024, 39, 11. DOI: 10.1016/j.jgar.2024.10.034.
2025
Byrnes, N. A. Applications of Chromatography and Mass Spectrometry for Advancing Public Health and Environmental Sustainability. Ph.D. Thesis, Monash University and the University of Bath, 2025. DOI: 10.26180/31329175.
This thesis presents research from international collaborations exploring applications of chromatography and mass spectrometry for advancing public health and environmental sustainability. Using liquid chromatography with tandem mass spectrometry, antimicrobial residues were quantified in wastewater from a national campaign, and identified a high risk of both antimicrobial resistance emergence and potential environmental harm. Hospitals were identified as a significant contributor of antimicrobials in wastewater further highlighting the need for comprehensive national action. Cannabinoids and pyrethrin pesticides, two naturally-occurring chemicals, were also examined using comprehensive two-dimensional gas chromatography with mass spectrometry to explore the unique capabilities, applications, and limitations of this technology.